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Expanding Expansion Microscopy to Reveal Hidden Nanostructures | Research & Technology | Sep 2022

Inside a living cell, proteins and other molecules are often tightly packed together. These dense clusters can be difficult to image because the fluor

Microscopy technique enables visualization of unseen nanostructures within cells and tissues

Inside a living cell, proteins and other molecules are often tightly packed together. These dense clusters can be difficult to image because the fluorescent labels used to make them visible can't wedge themselves in between the molecules.

Microscopy technique finds hidden nanostructures in cells and tissues

Microscopy technique finds hidden nanostructures in cells and tissues
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Microscopy technique reveals hidden nanostructures in cells and tissues

Microscopy technique reveals hidden nanostructures in cells and tissues
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Microscopy technique reveals hidden nanostructures in cells and tissues

MIT researchers developed a way to “de-crowd” molecules in a cell by expanding a tissue sample, labeling the molecules, then imaging them. The method, known as expansion revealing, builds on a technique known as expansion microscopy and should allow scientists to visualize molecules and cellular structures that have never been seen before.

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