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Microscopy technique makes finer images of deeper tissue, more quickly July 7, 2021MIT To create high-resolution, 3D images of tissues such as the brain, researchers often use two-photon microscopy, which involves aiming a high-intensity laser at the specimen to induce fluorescence excitation. However, scanning deep within the brain can be difficult because light scatters off of tissues as it goes deeper, making images blurry. Two-photon imaging is also time-consuming, as it usually requires scanning individual pixels one at a time. A team of MIT and Harvard University researchers has now developed a modified version of two-photon imaging that can image deeper within tissue and perform the imaging much more quickly than what was previously possible.

Murat yildirimCheng zhengJosiah boivinDushann wadduwageMriganka surYi xueNational institutes of healthImaging at harvard universityNational institute of biomedical imagingHamamatsu corporationHarvard universitySamsung advanced institute of technologyJohn harvard distinguished science fellowship programAlliance for researchJong kang parkJohn harvard distinguished science fellow