We reviewed the best dog toothpastes with help from vets. Our top recommended picks from Petsmile, Virbac, and Arm & Hammer include enzymatic toothpaste.
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Negative Control: Cellular system only
Treatment 1: Cellular system, Viral solution and Dental Gel APD (dilution without cytotoxic effect)
Treatment 2: Cellular system, Viral solution and Mouthwash APD (dilution without cytotoxic effect)
The determination of the virucidal activity of the product is carried out according to the Methodology TCID
50 (Median Tissue Culture Infectious Dose). The protocol considers the cultivation of the host cell lines of the virus – Vero ATCC cells
® CCL-81™ in culture microplates of 96 wells with subsequent dilution of the viral solution and the possible virucidal compound to be analyzed.
To evaluate the cytopathic effect, analyses are performed by means of an inverted microscope after an incubation period of 72 hours in an oven at 37°C with 5% CO