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DSCAM-AS1 and miR-122-5p. Although there has been a small amount of research on FSTL3, the exact mechanism by which FSTL3 functions as an oncogene remains unclear to date. Therefore, in the present study, we aimed to explore the molecular mechanism of FSTL3 involvement in gastric carcinogenesis and development, and to evaluate its value as a prognostic biomarker and potential therapeutic target for GC. Materials and Methods Cell Culture AGS (moderately differentiated GC cells), HGC-27 (undifferentiated GC cells), GES-1 (healthy gastric epithelial cells), and THP-1 cells (human monocytic cells) were purchased from the cell bank of the Chinese Academy of Sciences (Shanghai, China). MKN-74 and MKN-45 cells (well-differentiated and poorly differentiated GC cells, respectively) were purchased from the Japanese Collection of Research Bioresources Cell Bank. GC cells were cultured in RPMI-1640 medium (Gibco, USA, Lot: 8121248) supplemented with 10% fetal bovine serum (FBS) (Gibco, U

United statesUnited kingdomAmerican joint committee on cancerGraphpad software incAntibody sampler kit cell signaling technologyAaf associationChinese academy of sciences shanghaiJapanese collection of research bioresources cell bankGenechem corporation shanghaiColorful gene biological technologyNational comprehensive cancer networkChinese academyJapanese collectionResearch bioresources cellVector constructionGenechem corporation