Hop2-Mnd1 and Swi5-Sfr1 stimulate Dmc1-driven homologous recombination by acting on different steps during filament assembly, reveal Tokyo Tech researchers in collaboration with National Taiwan University. Using two types of single-molecule experimen
Decoding Dmc1 Filament Assembly in Homologous Recombination miragenews.com - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from miragenews.com Daily Mail and Mail on Sunday newspapers.
Homologous recombination (HR) is an important process that plays multiple crucial roles during meiosis, a type of cell cycle dedicated to sexual reproduction.
Unraveling the molecular basis of Dmc1 filament assembly in homologous recombination phys.org - get the latest breaking news, showbiz & celebrity photos, sport news & rumours, viral videos and top stories from phys.org Daily Mail and Mail on Sunday newspapers.
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IMAGE: CtIP/Ctp1 stimulates endonuclease activity of the MRN complex, which not only polishes dirty ends of DNA breaks, but promotes accurate repair of DNA breaks through the homologous recombination mechanism. view more
Credit: PNAS
Scientists at Tokyo Institute of Technology (Tokyo Tech) have uncovered mechanisms underlying the activation of the MRN complex the cell s DNA scissors. Using purified yeast proteins, they demonstrated that phosphorylation of Ctp1, a homolog of a tumor-suppressor protein, plays a key role in activating MRN complex s DNA clipping activity. Intriguingly, a short segment of yeast Ctp1 or its human counterpart could stimulate endonuclease activity of their respective MRN complexes, suggesting its conserved function across species.