In a bid to clinch the first title since 2018, Barcelona made a fine start to the contest, although only found a breakthrough in the 42nd minute when Alexia Putellas broke the deadlock from the spot.
Two minutes later, Jose Moncayo s side suffered a further setback when Aitana Bonmati struck to double the lead for Barcelona.
After the break, the champions continued their dominance and were rewarded with another breakthrough on the hour-mark when Leila Ouahabi played Jenni Hermoso through for the winner.
The latest Copa de la Reina title saw Barcelona surpass six-time winners Levante and Espanyol following a record seventh at Estadio La Rosaleda.