In this application note, Zymo Research emphasizes the significant impact of plasmid DNA quality on transfection success. Four key points are highlighted:
The DNA should be predominantly supercoiled and free of genomic DNA and RNA, as circular plasmids are more efficiently taken up by cells, while contamination confuses quantification.
The DNA should be highly concentrated and devoid of phenol, salts, and protein to prevent dilution of transfection reagents,