Review University Of South Florida Hyperbaric Biomedical Research Laboratory In hillsborough, florida, united-states | School In Hillsborough

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University Of South Florida Hyperbaric Biomedical Research Laboratory

Hillsborough,

Florida,United-states - 33612

Detailed description is To study the molecular and cellular mechanisms involved in the body’s response to artificial atmospheres and altered pressure environments.
The USF Hyperbaric Biomedical Research Laboratory is a collaborative research facility housed in the Department of Molecular Pharmacology and Physiology in the laboratories of Dr.
Jay B.
Dean: MDL 1027 and MDC 4042, 4046.
The HBRL maintains several hyperbaric/hypobaric pressure chambers containing equipment used to measure cellular function in real time via electrophysiology, polarography, fluorescence microscopy and atomic force microscopy during experimental perturbations of barometric pressure and gas partial pressures.
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The mission of the USF-HBRL is to identify the molecular and cellular mechanisms involved in the body’s response to artificial atmospheres and altered pressure environments, including low barometric pressures (hypobaria), normal sea level pressure (normobaria) and high barometric pressures (hyperbaria).
Altered pressure environments perturb various cellular processes at the molecular level due to the effects of pressure per se, gas partial pressure alone, and/or the production of secondary reaction productions such as O2-induced free radicals or CO2-induced protons.
Altered pressure environments are routinely encountered in hyperbaric medicine (hyperbaric oxygen therapy), underwater diving (hyperbaric gases) and space exploration (hypobaric gases).
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To study cellular processes under these conditions, Dr.
Dean and his colleagues at USF, Dr.
Enrico Camporesi (Chairman, USF Anesthesiology) and Dr.
Dominic D’Agostino (Molecular Pharmacology and Physiology), have assembled six hyperbaric/hypobaric pressure chambers for in vitro and in vivo biomedical research.
The design, development and implementation of these novel research tools have been funded primarily through equipment grants from the Department of Defense and Office of Naval Research (ONR), Undersea Medicine Program.
Ongoing basic research by the USF-HBRL team is currently supported by the ONR, NIH and USF College of Medicine.
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Recent publications.
D'Agostino, DP.Putnam, RW.Dean, JB.
Superoxide ({middle dot}O2 ) Production in CA1 Neurons of Rat Hippocampal Slices Exposed to Graded Levels of Oxygen.
Journal of neurophysiology.
98(2) : 1030-41, 2007.
http://www.ncbi.nlm.nih.gov/pubmed/17553943.
Dean, JB.
Metabolic acidosis inhibits hypothalamic warm-sensitive receptors: a potential causative factor in heat stroke.
Journal of applied physiology (Bethesda, Md.
: 1985).
102(4) : 1312, 2007.
http://www.ncbi.nlm.nih.gov/pubmed/17204570.
Ritucci, NA.Dean, JB.Putnam, RW.
Somatic vs.
dendritic responses to hypercapnia in chemosensitive locus coeruleus neurons from neonatal rats.
American journal of physiology.
Cell physiology.
289(5) : C1094-104, 2005.
http://www.ncbi.nlm.nih.gov/pubmed/16014703.
Mulkey, DK.Henderson, RA.Ritucci, NA.Putnam, RW.Dean, JB.
Oxidative stress decreases pHi and Na(+)/H(+) exchange and increases excitability of solitary complex neurons from rat brain slices.
American journal of physiology.
Cell physiology.
286(4) : C940-51, 2004.
http://www.ncbi.nlm.nih.gov/pubmed/14668260.
Dean, JB.Mulkey, DK.Henderson, RA.Potter, SJ.Putnam, RW.
Hyperoxia, reactive oxygen species, and hyperventilation: oxygen sensitivity of brain stem neurons.
Journal of applied physiology (Bethesda, Md.
: 1985).
96(2) : 784-91, 2004.
http://www.ncbi.nlm.nih.gov/pubmed/14715688.
Mulkey, DK.Henderson, RA.Putnam, RW.Dean, JB.
Hyperbaric oxygen and chemical oxidants stimulate CO2/H+-sensitive neurons in rat brain stem slices.
Journal of applied physiology (Bethesda, Md.
: 1985).
95(3) : 910-21, 2003.
http://www.ncbi.nlm.nih.gov/pubmed/12704094.
Dean, JB.Mulkey, DK.Garcia, AJ.Putnam, RW.Henderson, RA.
Neuronal sensitivity to hyperoxia, hypercapnia, and inert gases at hyperbaric pressures.
Journal of applied physiology (Bethesda, Md.
: 1985).
95(3) : 883-909, 2003.
http://www.ncbi.nlm.nih.gov/pubmed/12909594.
Mulkey, DK.Henderson, RA.Putnam, RW.Dean, JB.
Pressure (< or=4 ATA) increases membrane conductance and firing rate in the rat solitary complex.
Journal of applied physiology (Bethesda, Md.
: 1985).
95(3) : 922-30, 2003.
http://www.ncbi.nlm.nih.gov/pubmed/12704095.
Solomon, IC.Dean, JB.
Gap junctions in CO(2)-chemoreception and respiratory control.
Respiratory physiology & neurobiology.
131(3) : 155-73, 2002.
http://www.ncbi.nlm.nih.gov/pubmed/12126918.
Dean, JB.Ballantyne, D.Cardone, DL.Erlichman, JS.Solomon, IC.
Role of gap junctions in CO(2) chemoreception and respiratory control.
American journal of physiology.
Lung cellular and molecular physiology.
283(4) : L665-70, 2002.
http://www.ncbi.nlm.nih.gov/pubmed/12225940.
University of South Florida Hyperbaric Biomedical Research Laboratory is a private schoool located in , hillsborough of florida,united-states.

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