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D), (
E) Hydrodynamic diameters and Zeta potentials of cAuNPs and mAuNPs measured with dynamic light scattering (DLS). Note: hydrodynamic diameter and Zeta potential measurements are presented in the supporting information.
Cellular Uptake of mAuNPs
Shown in Figure 2A are photographs taken under dark field microscope of B16-F10 cells cultured with and without mAuNPs for 24 h. Numerous small spots in B16-F10 cells co-cultured with mAuNPs for 24 h were visible compared to controls, indicating that mAuNPs entered B16-F10 cells. Next, we investigated the cellular uptake of mAuNPs after B16-F10 cells were co-cultured with mAuNPs at different concentrations by ICP-AES (Figure 2B). The amount of cellular uptake of mAuNPs increased with the AuNPs co-culture concentrations in the range of ~0–60 μg/mL. The cellular uptake amount reached 3.6 pg/cell at the co-culture concentration of 10 μg/mL. If the weight of 10
FinlandChinaJapanUnited-statesWu-weiGansuSwitzerlandChineseBax-bclInstitute-of-modern-physicsNational-institutes-of-healthDSCAM-AS1 and miR-122-5p.
Although there has been a small amount of research on FSTL3, the exact mechanism by which
FSTL3 functions as an oncogene remains unclear to date. Therefore, in the present study, we aimed to explore the molecular mechanism of
FSTL3 involvement in gastric carcinogenesis and development, and to evaluate its value as a prognostic biomarker and potential therapeutic target for GC.
Materials and Methods
Cell Culture
AGS (moderately differentiated GC cells), HGC-27 (undifferentiated GC cells), GES-1 (healthy gastric epithelial cells), and THP-1 cells (human monocytic cells) were purchased from the cell bank of the Chinese Academy of Sciences (Shanghai, China). MKN-74 and MKN-45 cells (well-differentiated and poorly differentiated GC cells, respectively) were purchased from the Japanese Collection of Research Bioresources Cell Bank. GC cells were cultured in RPMI-1640 medium (Gibco, USA, Lot: 8121248) supplemented with 10% fetal bovine serum (FBS) (Gibco, U
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