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Escherichia coli and
Klebsiella), incubated at 37°C, under aerobic conditions, were also harvested for comparison purposes as negative controls, to exclude antigenic cross-reactivity with normal flora.
IGeneX Laboratories
Blood and skin samples were forwarded to IGeneX Laboratories, Milpitas, CA, for tickborne disease testing. Serological and PCR testing was performed as follows:
IGeneX Serology
Borrelia Western Blot (WB) Testing
The WB assays to detect IgM and IgG antibodies reactive to Bb and RFB were performed as previously described.
2,19 Nitrocellulose strips were prepared from whole-cell lysates of Bbss strains B31 and 297. Nitrocellulose strips for RFB WBs were prepared from lysates of
Borrelia hermsii and
35
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