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Genome-Wide Study of Off-Target Effects of Prime Editing in Rice Plants Demonstrates High Specificity April 22, 2021 The CRISPR-based genome editing toolbox is packed with powerful refinements. The latest of these, prime editing (PE), was first reported in Nature in 2019 by David Liu’s group. Using a prime editing guide RNA that identifies the genomic target site and a catalytically inactive Cas9 fused to reverse transcriptase, prime editing can introduce small insertions and deletions in addition to single base changes. Prime editors have been applied in animals and plants, but changes introduced at genomic sites other than the intended site (off-target effects) are a major hindrance in its application. Guide RNA (gRNA)-dependent off-target effects result from sequence similarities between the target site and other sites in the genome, whereas gRNA-independent off-target effects result from anomalous activity of CRISPR-based tools, such as deaminase, at non-target positions i

Gao caixiaDavid liuInstitute of geneticsChinese academy of sciencesChinese academyNature biotechnologyDevelopmental biologyடேவிட் லியூநிறுவனம் ஆஃப் ஜெநெடிக்ஸ்சீன கலைக்கழகம் ஆஃப் அறிவியல்சீன கலைக்கழகம்இயற்கை உயிரி தொழில்நுட்பவியல்வளர்ச்சி உயிரியல்