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+CD27 + B cells at each sampling time point (top). Mean frequencies of SARS-CoV-2 S-specific B cells expressing the indicated isotype in mild and severe donors (bottom). Error bars denote standard deviation and black bars indicate means. (F) Proportion of SARS-CoV-2 S-specific swIg + B cells that express CD71 at each sampling time point (top). Mean frequencies of SARS-CoV-2 S-specific swIg +CD71 + B cells in mild and severe donors (bottom). Error bars denote standard deviation and black bars indicate means. The dotted line indicates the level of CD71 expression on swIg + B cells in pre-pandemic donor samples. (G) Representative gating for SARS-CoV-2 S-specific B cells. The gated populations were single cell sorted for mAb cloning. Statistical comparisons between naive and convalescent donors were made by two-sided one-way Welsh ANOVA for unpaired samples with Dunnett’s T3 multiple comparisons test. Statistical comparisons between timepoints among convalescent donors were perfo

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