Moja Expressway a subsidiary of China Road and Bridge Construction (CRBC) will operate it for 27 years to recover its money before ceding the road to the State.
The government increased the charges to cushion the Chinese investor funding the road against exchange losses in the wake of the shilling weakening to record lows against the dollar.
Motorists will pay toll fees on both new and existing roads if MPs approve a law that paves the way for the introduction of user fees on major roads, bridges and tunnels.